The MRPL49 Antibody (PAC035278) is a crucial tool for researchers studying mitochondrial ribosomal protein L49 (MRPL49) in various biological processes. This polyclonal antibody, produced in rabbits, is highly specific to human samples and has been validated for Western blot applications. By binding to the MRPL49 protein, this antibody allows for precise and accurate detection and analysis in a variety of cell types.MRPL49 is an essential component of the mitochondrial ribosome, playing a key role in protein synthesis within the mitochondria. Dysregulation of MRPL49 has been implicated in various diseases, including cancer and neurodegenerative disorders.
Research into the function and regulation of MRPL49 is crucial for understanding the molecular mechanisms underlying these conditions and developing targeted therapies.With its high reactivity and specificity, the MRPL49 Antibody (PAC035278) is a valuable tool for scientists investigating mitochondrial protein synthesis and its implications in health and disease. Its versatility and reliability make it an excellent choice for studies in molecular biology, biochemistry, and medical research.
Western blot. All lanes: MRPL49 antibody at 0.6µg/ml. Lane 1: Colo320 whole cell lysate. Lane 2: Mouse liver tissue. Lane 3: Mouse kidney tissue. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 20 kDa. Observed band size: 20 kDa.
Immunohistochemistry of paraffin-embedded human colon tissue using PACO35278 at dilution of 1:100.
Immunohistochemistry of paraffin-embedded human kidney tissue using PACO35278 at dilution of 1:100.
Synonyms:
39S ribosomal protein L49, mitochondrial (L49mt) (MRP-L49) (Mitochondrial large ribosomal subunit protein mL49) (Neighbor of FAU) (NOF) (Protein NOF1), MRPL49, C11orf4 NOF1
UniProt Protein Function:
MRPL49: a mitochondrial ribosomal protein encoded by a nuclear gene that helps in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. Pseudogenes corresponding to this gene are found on chromosomes 5q and 8p. [provided by RefSeq, May 2011]Protein type: Ribosomal; Translation; MitochondrialChromosomal Location of Human Ortholog: 11q13Cellular Component: mitochondrial inner membrane; mitochondrial large ribosomal subunit; mitochondrial ribosomeMolecular Function: protein binding; structural constituent of ribosomeBiological Process: mitochondrial translation; organelle organization and biogenesis
UniProt Protein Details:
NCBI Summary:
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. Pseudogenes corresponding to this gene are found on chromosomes 5q and 8p. [provided by RefSeq, May 2011]
