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Mouse Protein Disulfide Isomerase A2 (PDIA2) ELISA Kit

SKU:
MODL01025
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
D3Z6P0
Sensitivity:
0.058ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
PDI-A2,PDA2, PDI, PDIR,PDIP,Protein Disulfide Isomerase,Pancreatic,Protein Disulfide Isomerase-Associated 2,Pancreas-specific protein disulfide isomerase
Reactivity:
Mouse
Sample Type:
Tissue Homogenates And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Mouse Protein Disulfide Isomerase A2 (PDIA2) ELISA Kit

The Mouse Protein Disulfide Isomerase A2 (PDIA2) ELISA Kit is specially designed for the accurate detection of PDIA2 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.PDIA2 is an important protein involved in protein folding and quality control within the cell.

It plays a key role in maintaining cellular homeostasis and is implicated in many diseases such as cancer, diabetes, and neurodegenerative disorders. This ELISA kit provides a valuable tool for studying the function of PDIA2 and its potential implications in various pathological conditions.

Product Name:Mouse Protein Disulfide Isomerase A2 (PDIA2) ELISA Kit
Product Code:MODL01025
Size:96 Assays
Target:PDIA2
Synonyms:PDI-A2, PDA2, PDI, PDIR, PDIP, Protein Disulfide Isomerase,Pancreatic, Protein Disulfide Isomerase-Associated 2, Pancreas-specific protein disulfide isomerase
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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