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Mouse Mannose Receptor C Type 2 (MRC2) ELISA Kit

SKU:
MODL00888
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q64449
Sensitivity:
0.054ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
CD280,ENDO180,CLEC13E,UPARAP,C-type lectin domain family 13 member E,Endocytic receptor 180,Macrophage mannose receptor 2,Urokinase receptor-associated protein
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Mouse Mannose Receptor C Type 2 (MRC2) ELISA Kit

The Mouse Mannose Receptor C-Type 2 (MRC2) ELISA Kit is a powerful tool for accurately measuring levels of MRC2 in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.MRC2 is a key protein involved in the immune response and cellular signaling pathways. Its expression has been implicated in various diseases, including cancer, autoimmune disorders, and inflammation.

By monitoring MRC2 levels, researchers can gain valuable insights into disease mechanisms and potential therapeutic targets.The Mouse Mannose Receptor C-Type 2 (MRC2) ELISA Kit is a valuable asset for studying the role of MRC2 in disease pathology and developing novel treatment strategies. Its reliability and accuracy make it an essential tool for researchers in the field of immunology and molecular biology.

Product Name:Mouse Mannose Receptor C Type 2 (MRC2) ELISA Kit
Product Code:MODL00888
Size:96 Assays
Target:MRC2
Synonyms:CD280, ENDO180, CLEC13E, UPARAP, C-type lectin domain family 13 member E, Endocytic receptor 180, Macrophage mannose receptor 2, Urokinase receptor-associated protein
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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