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Mouse Interleukin 1 Beta (IL-1b) ELISA Kit

SKU:
MODL00690
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P10749
Sensitivity:
6.2pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
IL1-B,IL1-Beta,IL1F2,IL-1Œ?,Interleukin-1 Family Member 2,Catabolin
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€599
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Description

system_update_altDatasheet

Mouse Interleukin 1 Beta (IL-1b) ELISA Kit

The Mouse Interleukin-1 Beta (IL1B) ELISA Kit is a powerful tool for the precise quantification of IL1B levels in mouse samples including serum, plasma, and cell culture supernatants. This kit is known for its exceptional sensitivity and specificity, guaranteeing accurate and consistent results for various research applications.IL1B is a key cytokine that plays a critical role in inflammation and immune response regulation. Elevated levels of IL1B have been linked to a number of diseases such as inflammatory disorders, autoimmune diseases, and infectious diseases.

By accurately measuring IL1B levels, researchers can gain valuable insights into the pathogenesis of these conditions and explore potential therapeutic interventions.Overall, the Mouse Interleukin-1 Beta (IL1B) ELISA Kit is an essential tool for researchers studying the role of IL1B in disease mechanisms and therapeutic targeting, providing reliable data to advance their research efforts.

Product Name:Mouse Interleukin 1 Beta (IL-1b) ELISA Kit
Product Code:MODL00690
Size:96 Assays
Target:IL1b
Synonyms:IL1-B, IL1-Beta, IL1F2, IL-1?, Interleukin-1 Family Member 2, Catabolin
Detection Method:Sandwich
Range:15.625-1000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-10396
Heparin plasma (n=5)89-10295
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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