The Mouse Histone H2B ELISA Kit is meticulously engineered to facilitate the accurate quantification of histone H2B levels in mouse biological samples. Histone H2B, a fundamental component of chromatin structure, plays a crucial role in gene regulation, chromatin integrity, and epigenetic modifications. As a core histone protein, fluctuations in histone H2B levels can impact gene expression patterns, DNA packaging, and overall chromatin function, influencing cellular processes and contributing to various physiological and pathological conditions. Utilizing this ELISA kit, researchers can precisely measure histone H2B levels, offering valuable insights into epigenetic regulation, chromatin dynamics, and gene expression modulation.
The quantitative analysis of histone H2B is instrumental in elucidating epigenetic mechanisms, studying chromatin organization, and investigating the role of histone modifications in transcriptional regulation and cellular functions. With exceptional sensitivity and specificity, the Mouse Histone H2B ELISA Kit provides reliable and reproducible results, empowering researchers to explore histone biology and epigenetic processes with confidence. Manufactured under rigorous quality control standards, this kit ensures robust performance and ease of use, making it an indispensable tool for investigations into chromatin structure, gene regulation, and epigenetic modifications in mouse models.
Product Name:
Mouse histon-H2b ELISA Kit
SKU:
AEES00676
Target:
Mouse histon-H2b
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
4.5h
Sensitivity:
0.23 ng/mL
Detection range:
0.39-25 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse histon-H2b. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse histon-H2b and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse histon-H2b, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse histon-H2b. You can calculate the concentration of Mouse histon-H2b in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-110
86-100
96-113
Average (%)
100
92
104
1:4
Range (%)
88-100
81-94
87-100
Average (%)
93
88
92
1:8
Range (%)
93-105
83-96
82-97
Average (%)
99
89
89
1:16
Range (%)
89-102
86-100
88-100
Average (%)
96
92
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-102
94
EDTA plasma (n=5)
89-105
97
Cell culture media (n=5)
88-102
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.15
2.86
10.68
1.21
2.92
11.21
Standard deviation
0.07
0.13
0.58
0.08
0.13
0.37
C V (%)
6.09
4.55
5.43
6.61
4.45
3.3
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse histon-H2b concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse histon-H2b in samples. No significant cross-reactivity or interference between Mouse histon-H2b and analogues was observed.
