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Mouse Colony Stimulating Factor 3, Granulocyte (GCSF) ELISA Kit

SKU:
MODL00535
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P09920
Sensitivity:
5.1pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
CSF3,G-CSF,Granulocyte Colony Stimulating Factor,Pluripoietin,Filgrastim,Lenograstim
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€599
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Description

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Mouse Colony Stimulating Factor 3, Granulocyte (GCSF) ELISA Kit

The Mouse Colony Stimulating Factor 3 (Granulocyte GCSF) ELISA Kit is specifically designed for the precise detection of Granulocyte Colony Stimulating Factor 3 (GCSF) levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for various research applications.GCSF is a vital cytokine that plays a key role in stimulating the production and function of granulocytes, essential components of the immune system.

It is involved in regulating immune responses and inflammation, making it a valuable biomarker for the study of immune-related diseases, infections, and inflammatory conditions.With this ELISA kit, researchers can effectively measure GCSF levels in mouse samples, allowing for in-depth investigation into its role in immune system function and its potential as a therapeutic target. Get reliable and insightful results with the Mouse Colony Stimulating Factor 3 (Granulocyte GCSF) ELISA Kit from Assay Genie.

Product Name:Mouse Colony Stimulating Factor 3, Granulocyte (GCSF) ELISA Kit
Product Code:MODL00535
Size:96 Assays
Target:GCSF
Synonyms:CSF3, G-CSF, Granulocyte Colony Stimulating Factor, Pluripoietin, Filgrastim, Lenograstim
Detection Method:Sandwich
Range:15.625-1,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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