The Monomethyl Histone H3 R2 Polyclonal Antibody (CAB3154) is a valuable tool for researchers studying epigenetics and chromatin remodeling. This antibody, generated in rabbits, is highly specific in detecting the monomethylated form of Histone H3 at Arginine 2 (H3R2me1) in human samples. Validated for use in Western blot applications, this antibody allows for the precise detection and analysis of histone modifications in various cell types.Histone modifications, such as methylation of specific amino acid residues, play a crucial role in regulating gene expression and chromatin structure. The monomethylation of Histone H3 at Arginine 2 is associated with transcriptional activation and is involved in processes like DNA repair and cell differentiation.
By targeting this specific histone modification, researchers can gain insights into gene regulation mechanisms and cellular functions.The Monomethyl Histone H3 R2 Polyclonal Antibody is an essential tool for investigating epigenetic changes associated with disease states, such as cancer and developmental disorders. Understanding the role of histone modifications like H3R2me1 in these conditions can lead to the development of targeted therapies that modulate gene expression. With its high specificity and sensitivity, this antibody is ideal for studies in epigenetics, cancer biology, and stem cell research.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Purification Method:
Affinity purification
Gene ID:
8290/8350
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using MonoMethyl-Histone H3-R2 Rabbit pAb (CAB3154) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 20s.
