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Monkey Transforming Growth Factor Alpha (TGFa) ELISA Kit

SKU:
MKDL00030
Product Type:
ELISA Kit
Sensitivity:
8.4pg/mL
Range:
31.25-2000pg/mL
ELISA Type:
Sandwich
Synonyms:
ETGF,TGF-A,Protransforming Growth Factor alpha,EGF-Like TGF
Reactivity:
Monkey
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Monkey Transforming Growth Factor Alpha (TGFa) ELISA Kit

The Monkey Transforming Growth Factor Alpha (TGFA) ELISA Kit is a highly sensitive and specific assay designed for the accurate detection of TGFA levels in monkey serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a variety of research applications.TGFA is a key protein involved in cell growth and differentiation, particularly in the context of tissue regeneration and wound healing.

Dysregulation of TGFA expression has been implicated in various disease processes, including cancer and inflammatory disorders. As such, the Monkey TGFA ELISA Kit is an essential tool for studying the role of TGFA in health and disease, as well as for developing potential therapeutic interventions.

Product Name:Monkey Transforming Growth Factor Alpha (TGFa) ELISA Kit
Product Code:MKDL00030
Size:96 Assays
Target:TGFa
Synonyms:ETGF, TGF-A, Protransforming Growth Factor alpha, EGF-Like TGF
Detection Method:Sandwich
Range:31.25-2000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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