The MOCS2 Antibody (PAC060945) is a highly specific and sensitive tool for researchers studying MOCS2, a protein involved in molybdenum cofactor biosynthesis. This polyclonal antibody, generated in rabbits, is optimized for detection of MOCS2 in human samples and is validated for use in applications such as Western blotting. By targeting the MOCS2 protein, this antibody allows for precise analysis and localization of MOCS2 in various cell types, making it an invaluable tool for studies in biochemistry and molecular biology.MOCS2 is a key enzyme in the biosynthesis of the molybdenum cofactor, which is essential for the activity of several enzymes involved in cellular metabolism.
Dysregulation of MOCS2 expression or function can lead to severe metabolic disorders, making it a crucial target for research into rare genetic diseases and disorders of molybdenum metabolism. The MOCS2 Antibody provides researchers with a reliable means to investigate the role of MOCS2 in health and disease, furthering our understanding of molybdenum cofactor biosynthesis and related disorders.
Antibody Name:
MOCS2 Antibody (PACO60945)
Antibody SKU:
PACO60945
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, IF
Recommended Dilutions:
ELISA:1:2000-1:10000, IF:1:50-1:200
Species Reactivity:
Human
Immunogen:
Recombinant Human Molybdopterin synthase sulfur carrier subunit protein (1-88AA)
Immunofluorescence staining of HepG2 cells with PACO60945 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
Acts as a sulfur carrier required for molybdopterin biosynthesis. Component of the molybdopterin synthase complex that catalyzes the conversion of precursor Z into molybdopterin by mediating the incorporation of 2 sulfur atoms into precursor Z to generate a dithiolene group. In the complex, serves as sulfur donor by being thiocarboxylated (-COSH) at its C-terminus by MOCS3. After interaction with MOCS2B, the sulfur is then transferred to precursor Z to form molybdopterin.
Synonyms:
Molybdopterin synthase sulfur carrier subunit (MOCO1-A) (Molybdenum cofactor synthesis protein 2 small subunit) (Molybdenum cofactor synthesis protein 2A) (MOCS2A) (Molybdopterin-synthase small subunit) (Sulfur carrier protein MOCS2A), MOCS2, MOCO1
UniProt Protein Function:
Catalytic subunit of the molybdopterin synthase complex, a complex that catalyzes the conversion of precursor Z into molybdopterin. Acts by mediating the incorporation of 2 sulfur atoms from thiocarboxylated MOCS2A into precursor Z to generate a dithiolene group.
NCBI Summary:
Eukaryotic molybdoenzymes use a unique molybdenum cofactor (MoCo) consisting of a pterin, termed molybdopterin, and the catalytically active metal molybdenum. MoCo is synthesized from precursor Z by the heterodimeric enzyme molybdopterin synthase. The large and small subunits of molybdopterin synthase are both encoded from this gene by overlapping open reading frames. The proteins were initially thought to be encoded from a bicistronic transcript. They are now thought to be encoded from monocistronic transcripts. Alternatively spliced transcripts have been found for this locus that encode the large and small subunits. [provided by RefSeq, Jul 2008]
