The MMP15 Polyclonal Antibody (PACO14710) is a valuable tool for researchers studying MMP15, a matrix metalloproteinase enzyme involved in extracellular matrix degradation and tissue remodeling. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to MMP15 protein, this antibody enables precise detection and analysis in a variety of cell types, making it ideal for investigations in fields such as cancer biology and tissue biology. MMP15, also known as membrane-type matrix metalloproteinase 15, plays a crucial role in processes like cell migration, invasion, and angiogenesis by cleaving various extracellular matrix components.
Its dysregulation has been implicated in diseases like cancer, cardiovascular disorders, and neurological conditions, making it a promising target for therapeutic interventions. Understanding the functions and regulation of MMP15 is essential for developing strategies to modulate its activity and potentially combat pathological conditions associated with its aberrant expression.
Antibody Name:
MMP15 Antibody (PACO14710)
Antibody SKU:
PACO14710
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:5000, WB:1:500-1:2000
Species Reactivity:
Human, Mouse
Immunogen:
Fusion protein of human MMP15
Form:
Liquid
Storage Buffer:
-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:
Antigen affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-4: Mouse stomach tissue, Mouse heart tissue, Mouse liver tissue, HT29 cells, Primary antibody: PACO14710(MMP15 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute.
Background:
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted.
Synonyms:
matrix metallopeptidase 15 (membrane-inserted)
UniProt Protein Function:
MMP15: Endopeptidase that degrades various components of the extracellular matrix. May activate progelatinase A. Belongs to the peptidase M10A family.
UniProt Protein Details:
Protein type:Membrane protein, integral; EC 3.4.24.-; Protease
Chromosomal Location of Human Ortholog: 16q13
Cellular Component: plasma membrane
Molecular Function:enzyme activator activity; protein binding; serine-type endopeptidase activity
Biological Process: collagen catabolic process; extracellular matrix disassembly; protein modification process
NCBI Summary:
This gene encodes a member of the peptidase M10 family and membrane-type subfamily of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Members of this subfamily contain a transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. The encoded preproprotein is proteolytically processed to generate the mature protease. This protein may play a role in cancer progression. [provided by RefSeq, Jan 2016]
