The MLH3 Polyclonal Antibody (CAB7796) is a valuable tool for researchers studying MLH3, a key protein involved in DNA mismatch repair. This antibody, raised in rabbits, shows high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the MLH3 protein, this antibody enables precise detection and analysis in a variety of cell types, making it an ideal choice for studies in genetics, cancer research, and molecular biology.MLH3 is a crucial component of the mismatch repair system, which plays a vital role in maintaining genomic stability and preventing mutations that can lead to cancer and other genetic disorders.
Research on MLH3 can provide valuable insights into the mechanisms underlying DNA repair processes and the development of therapeutic strategies for diseases related to DNA damage.The MLH3 Polyclonal Antibody offers researchers a reliable tool for investigating the function and regulation of MLH3, shedding light on its role in DNA repair pathways and its potential as a target for novel cancer therapies. By using this antibody, scientists can deepen their understanding of the molecular mechanisms underlying genetic stability and disease development.
Product Name:
MLH3 Rabbit Polyclonal Antibody
SKU:
CAB7796
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1190-1389 of human MLH3 (NP_055196.2).
This gene is a member of the MutL-homolog (MLH) family of DNA mismatch repair (MMR) genes. MLH genes are implicated in maintaining genomic integrity during DNA replication and after meiotic recombination. The protein encoded by this gene functions as a heterodimer with other family members. Somatic mutations in this gene frequently occur in tumors exhibiting microsatellite instability, and germline mutations have been linked to hereditary nonpolyposis colorectal cancer type 7 (HNPCC7). Several alternatively spliced transcript variants have been identified, but the full-length nature of only two transcript variants has been determined.
Purification Method:
Affinity purification
Gene ID:
27030
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of HCT116, using MLH3 Rabbit pAb (CAB7796) at 1:3500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
