The MID1 Polyclonal Antibody (CAB7291) is an essential tool for researchers studying MID1, a protein involved in cellular functions such as protein ubiquitination and gene expression regulation. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By targeting the MID1 protein, this antibody enables precise detection and analysis in various cell types, making it a valuable tool for studies in cell biology, developmental biology, and cancer research.MID1, a ubiquitin ligase protein, plays a crucial role in various cellular processes, including cell growth, differentiation, and survival.
Dysregulation of MID1 has been linked to developmental disorders, cancer, and other diseases, making it a promising target for therapeutic interventions. Understanding the functions and regulatory mechanisms of MID1 is essential for advancing our knowledge of cellular processes and identifying potential targets for drug development.Overall, the MID1 Polyclonal Antibody (CAB7291) offers researchers a reliable and specific tool for investigating the roles of MID1 in cellular processes, providing valuable insights into the molecular mechanisms underlying various diseases and informing the development of novel therapeutic strategies.
Product Name:
MID1 Rabbit Polyclonal Antibody
SKU:
CAB7291
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 478-667 of human MID1 (NP_000372.1).
The protein encoded by this gene is a member of the tripartite motif (TRIM) family, also known as the 'RING-B box-coiled coil' (RBCC) subgroup of RING finger proteins. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein forms homodimers which associate with microtubules in the cytoplasm. The protein is likely involved in the formation of multiprotein structures acting as anchor points to microtubules. Mutations in this gene have been associated with the X-linked form of Opitz syndrome, which is characterized by midline abnormalities such as cleft lip, laryngeal cleft, heart defects, hypospadias, and agenesis of the corpus callosum. This gene was also the first example of a gene subject to X inactivation in human while escaping it in mouse. Alternative promoter use, alternative splicing and alternative polyadenylation result in multiple transcript variants that have different tissue specificities.
Purification Method:
Affinity purification
Gene ID:
4281
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using MID1 Rabbit pAb (CAB7291) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
