The Mevalonate Kinase Polyclonal Antibody (CAB5354) is a valuable tool for researchers studying the mevalonate pathway, a critical metabolic pathway involved in cholesterol and isoprenoid biosynthesis. This antibody, generated in rabbits, exhibits high specificity and sensitivity for detecting mevalonate kinase in human samples, making it an ideal choice for Western blot applications.Mevalonate kinase is an essential enzyme in the mevalonate pathway, playing a key role in the production of important cellular components such as cholesterol, steroids, and isoprenoids. Dysregulation of this pathway has been linked to various diseases, including cancer and metabolic disorders.
Studying the activity and expression of mevalonate kinase is therefore crucial for understanding these disease processes and identifying potential therapeutic targets.Whether investigating lipid metabolism, cell signaling, or the pathogenesis of diseases, the Mevalonate Kinase Polyclonal Antibody (CAB5354) offers a reliable tool for researchers seeking to delve deeper into the intricate workings of the mevalonate pathway. Its high specificity and sensitivity ensure accurate detection of mevalonate kinase in a variety of experimental settings, making it a valuable asset for studies in biochemistry, pharmacology, and beyond.
Product Name:
MVK Rabbit Polyclonal Antibody
SKU:
CAB5354
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 40-300 of human MVK (NP_000422.1).
This gene encodes the peroxisomal enzyme mevalonate kinase. Mevalonate is a key intermediate, and mevalonate kinase a key early enzyme, in isoprenoid and sterol synthesis. Mevalonate kinase deficiency caused by mutation of this gene results in mevalonic aciduria, a disease characterized psychomotor retardation, failure to thrive, hepatosplenomegaly, anemia and recurrent febrile crises. Defects in this gene also cause hyperimmunoglobulinaemia D and periodic fever syndrome, a disorder characterized by recurrent episodes of fever associated with lymphadenopathy, arthralgia, gastrointestinal dismay and skin rash. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
4598
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using MVK antibody (CAB5354) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
