The MECP2 Polyclonal Antibody (CAB5694) is an essential tool for researchers studying MECP2, a protein that plays a critical role in neurodevelopment and gene expression regulation. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in various applications, including Western blotting.MECP2, also known as methyl-CpG-binding protein 2, is a key player in the maintenance of genomic methylation patterns and the regulation of gene expression. Dysregulation of MECP2 has been linked to neurodevelopmental disorders, including Rett Syndrome.
Therefore, investigating the function and expression levels of MECP2 is crucial for understanding its role in neurological health and disease.By utilizing the MECP2 Polyclonal Antibody, researchers can detect and analyze MECP2 protein levels in different cell types, providing valuable insights into its mechanisms of action. This antibody is a valuable tool for studies in neuroscience, developmental biology, and genetic disorders associated with MECP2 dysfunction.
Product Name:
MECP2 Rabbit Polyclonal Antibody
SKU:
CAB5694
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 387-486 of human MECP2 (NP_004983.1).
DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of most cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of cognitive disability in females. Alternative splicing results in multiple transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
4204
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using MECP2 Rabbit pAb (CAB5694) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates / proteins: 25 μg per lane.Blocking buffer: 3 % nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 20s.
