The MCM9 Polyclonal Antibody (PAC041734) is a valuable tool for researchers studying MCM9, a protein involved in DNA replication and repair processes. This antibody, produced in rabbits, demonstrates high reactivity with human samples and has been validated for use in Western blot applications. By targeting the MCM9 protein, this antibody allows for the detection and analysis of MCM9 in various cell types, making it an essential component for studies in molecular biology and cancer research.MCM9 is a key player in ensuring the fidelity of DNA replication and maintaining genomic stability.
Dysregulation of MCM9 has been associated with various human diseases, including cancer and genetic disorders. By investigating the functions and interactions of MCM9, researchers can gain insight into the mechanisms underlying these conditions and potentially identify new therapeutic targets for intervention.Overall, the MCM9 Polyclonal Antibody offers a powerful tool for researchers seeking to unravel the complexities of DNA replication and repair pathways, with implications for advancing our understanding of disease processes and developing targeted treatments.
Western blot. All lanes: MCM9 antibody at 0.8µg/ml. Lane 1: Mouse brain tissue. Lane 2: 293T whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 128, 73, 44, 85 kDa. Observed band size: 128 kDa.
Immunohistochemistry of paraffin-embedded human testis tissue using PACO41734 at dilution of 1:100.
Immunohistochemistry of paraffin-embedded human kidney tissue using PACO41734 at dilution of 1:100.
Background:
Component of the MCM8-MCM9 complex, a complex involved in homologous recombination repair following DNA interstrand cross-links and plays a key role during gametogenesis. The MCM8-MCM9 complex probably acts as a hexameric helicase downstream of the Fanconi anemia proteins BRCA2 and RAD51 and is required to process aberrant forks into homologous recombination substrates and to orchestrate homologous recombination with resection, fork stabilization and fork restart.
Synonyms:
DNA helicase MCM9 (hMCM9) (EC 3.6.4.12) (Mini-chromosome maintenance deficient domain-containing protein 1) (Minichromosome maintenance 9), MCM9, C6orf61 MCMDC1
UniProt Protein Function:
MCM9: Component of the MCM8-MCM9 complex, a complex involved in homologous recombination repair following DNA interstrand cross-links and plays a key role during gametogenesis. The MCM8- MCM9 complex probably acts as a hexameric helicase downstream of the Fanconi anemia proteins BRCA2 and RAD51 and is required to process aberrant forks into homologous recombination substrates and to orchestrate homologous recombination with resection, fork stabilization and fork restart. Belongs to the MCM family. 3 isoforms of the human protein are produced by alternative splicing.Protein type: EC 3.6.4.12Chromosomal Location of Human Ortholog: 6q22.31Cellular Component: nucleusMolecular Function: ATP binding; DNA binding; helicase activityBiological Process: DNA replication; double-strand break repair via homologous recombination; female gamete generation; response to DNA damage stimulusDisease: Ovarian Dysgenesis 4
UniProt Protein Details:
NCBI Summary:
The protein encoded by this gene is a member of the mini-chromosome maintenance (MCM) protein family that are essential for the initiation of eukaryotic genome replication. Binding of this protein to chromatin has been shown to be a pre-requisite for recruiting the MCM2-7 helicase to DNA replication origins. This protein also binds, and is a positive regulator of, the chromatin licensing and DNA replication factor 1, CDT1. [provided by RefSeq, Nov 2010]
