The LOXL1 Monoclonal Antibody is a valuable tool for studying LOXL1, an enzyme involved in the crosslinking of collagen and elastin in the extracellular matrix. This antibody, produced through hybridoma technology, demonstrates high specificity and sensitivity for detecting LOXL1 in various experimental settings.LOXL1 is known to play a crucial role in tissue remodeling and maintenance of structural integrity in connective tissues. Its dysregulation has been implicated in various diseases, including fibrosis and cancer.
Researchers studying LOXL1 can benefit from using this monoclonal antibody in techniques such as immunohistochemistry and flow cytometry to investigate its expression and function in different cell types and tissues.With its ability to specifically target LOXL1, this monoclonal antibody offers researchers a reliable tool for exploring the molecular mechanisms underlying tissue remodeling processes and potential therapeutic targets for diseases characterized by aberrant extracellular matrix remodeling.
Product Name:
LOXL1 Monoclonal Antibody
SKU:
CAB23079
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant protein of human LOXL1.
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:1000
Synonyms:
LOL; LOXL; LOXL1
Positive Sample:
U-87MG
Conjugate:
Unconjugated
Cellular Localization:
Secreted, extracellular space.
Calculated MW:
63kDa
Observed MW:
63kDa
This gene encodes a member of the lysyl oxidase family of proteins. The prototypic member of the family is essential to the biogenesis of connective tissue, encoding an extracellular copper-dependent amine oxidase that catalyzes the first step in the formation of crosslinks in collagen and elastin. The encoded preproprotein is proteolytically processed to generate the mature enzyme. A highly conserved amino acid sequence at the C-terminus end appears to be sufficient for amine oxidase activity, suggesting that each family member may retain this function. The N-terminus is poorly conserved and may impart additional roles in developmental regulation, senescence, tumor suppression, cell growth control, and chemotaxis to each member of the family. Mutations in this gene are associated with exfoliation syndrome.
Purification Method:
Affinity purification
Gene ID:
4016
Clone Number:
ARC58135
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates, using LOXL1 antibody (CAB23079) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
