The LDL Receptor (LDLR) Polyclonal Antibody is a valuable tool for researchers studying the LDL receptor, a key protein involved in cholesterol metabolism and cardiovascular health. This antibody, produced in rabbits, is highly specific to human samples and is suitable for use in Western blotting and immunohistochemistry applications.The LDL receptor is responsible for regulating levels of low-density lipoprotein (LDL) cholesterol in the blood by binding to LDL particles and facilitating their uptake into cells. Dysregulation of LDL receptor function is associated with conditions such as familial hypercholesterolemia and atherosclerosis, making it an important target for therapeutic development.
By using the LDL Receptor Polyclonal Antibody, researchers can accurately detect and analyze LDL receptor expression in various cell types and tissues. This antibody enables detailed investigation into the role of the LDL receptor in cholesterol metabolism, cardiovascular disease, and related disorders, providing valuable insights for the development of new treatments and interventions.
Product Name:
LDL Receptor (LDLR) Polyclonal Antibody
SKU:
CAB21806
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 761-860 of human LDL Receptor (LDLR) (NP_000518.1).
Cell membrane, Cell surface, Early endosome, Endomembrane system, Golgi apparatus, Late endosome, Lysosome, Membrane, Single-pass type I membrane protein, clathrin-coated pit.
Calculated MW:
95kDa
Observed MW:
100-160kDa
The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. The encoded protein is normally bound at the cell membrane, where it binds low density lipoprotein/cholesterol and is taken into the cell. Lysosomes release the cholesterol, which is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in this gene cause the autosomal dominant disorder, familial hypercholesterolemia. Alternate splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
3949
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates, using LDL Receptor (LDLR) Rabbit pAb (CAB21806) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
