LC3B Polyclonal Antibody (CAB11923)
- SKU:
- CAB11923
- Product Type:
- Antibody
- Antibody Type:
- Polyclonal Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- WB
- IHC
- IF
- IP
Frequently bought together:
Description
| Product Name: | LC3B Polyclonal Antibody |
| SKU: | CAB11923 |
| Size: | 20uL, 100uL |
| Isotype: | IgG |
| Host Species: | Rabbit |
| Reactivity: | Human,Mouse,Rat |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human LC3B (NP_073729.1). |
| Sequence: | MPSE KTFK QRRT FEQR VEDV RLIR EQHP TKIP VIIE RYKG EKQL PVLD KTKF LVPD HVNM SELI KIIR RRLQ LNAN QAFF LLVN GHSM VSVS TPIS EVYE |
| Tested Applications: | WB IHC-P IF/ICC ELISA |
| Recommended Dilution: | WB,1:500 - 1:2000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:100 |
| Synonyms: | LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3 |
| Positive Sample: | 293 |
| Conjugate: | Unconjugated |
| Cellular Localization: | Cytoplasm, Cytoplasmic vesicle, Endomembrane system, Lipid-anchor, autophagosome, autophagosome membrane, cytoskeleton. |
| Calculated MW: | 15kDa |
| Observed MW: | 20kDa |
The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.
| Purification Method: | Affinity purification |
| Gene ID: | 81631 |
| Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3. |
 | Western blot analysis of lysates from 293 cells, using LC3B Rabbit pAb (CAB11923).Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST. |
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