The LAMA5 Polyclonal Antibody (PAC018135) is a valuable tool for researchers studying LAMA5, a key component of the extracellular matrix implicated in cell adhesion and migration. This antibody, produced in rabbits, shows high reactivity towards human samples and has been validated for use in various applications, including Western blotting. By binding specifically to the LAMA5 protein, researchers can easily detect and analyze this important molecule in different cell types, making it an ideal choice for studies in cell biology and cancer research.
LAMA5, also known as laminin alpha 5, plays a critical role in maintaining tissue structure and function, particularly in the context of cell adhesion and migration processes. Its involvement in cell signaling pathways and tissue organization make it a target of interest in research on various diseases, including cancer and developmental disorders. Understanding the function of LAMA5 is essential for uncovering its role in health and disease, paving the way for potential therapeutic interventions targeting this molecule.
Antibody Name:
LAMA5 Antibody (PACO18135)
Antibody SKU:
PACO18135
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:1000-1:2000, IHC:1:10-1:50
Species Reactivity:
Human
Immunogen:
Synthetic peptide of human LAMA5
Form:
Liquid
Storage Buffer:
-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:
Antigen affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using PACO18135(LAMA5 Antibody) at dilution 1/8, on the right is treated with synthetic peptide. (Original magnification: x200).
Background:
Components of the extracellular matrix exert myriad effects on tissues throughout the body. In particular, the laminins, a family of heterotrimeric extracellular glycoproteins, affect tissue development and integrity in such diverse organs as the kidney, lung, skin, and nervous system. It is thought that laminins mediate the attachment, migration, and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Laminins function as heterotrimeric complexes of alpha, beta, and γ chains, with each chain type representing a different subfamily of proteins. The protein encoded by this gene belongs to the α subfamily of laminin chains and is a major component of basement membranes. Two transcript variants encoding different isoforms have been found for this gene, but the full-length nature of one of them has not been determined.
Synonyms:
laminin, α 5
UniProt Protein Function:
LAMA5: Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
UniProt Protein Details:
Protein type:Secreted, signal peptide; Secreted; Extracellular matrix; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 20q13.2-q13.3
Biological Process: integrin-mediated signaling pathway; regulation of cell adhesion; focal adhesion formation; endothelial cell differentiation; extracellular matrix organization and biogenesis; muscle development; cell migration; regulation of embryonic development; cell recognition; cytoskeleton organization and biogenesis; regulation of cell migration; regulation of cell proliferation; odontogenesis of dentine-containing teeth; cell proliferation; extracellular matrix disassembly; embryonic development; morphogenesis of embryonic epithelium; hair follicle development; ureteric bud branching; cilium biogenesis; angiogenesis; cell differentiation; neural crest cell migration; morphogenesis of a polarized epithelium; lung development
NCBI Summary:
This gene encodes one of the vertebrate laminin alpha chains. Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. The protein encoded by this gene is the alpha-5 subunit of of laminin-10 (laminin-511), laminin-11 (laminin-521) and laminin-15 (laminin-523). [provided by RefSeq, Jun 2013]
