The KO Validated NF-kB p65 Rabbit Monoclonal Antibody (CAB19653) is a powerful tool for research into NF-kB signaling pathways. NF-kB p65 is a key transcription factor involved in the regulation of inflammatory and immune responses, making this antibody essential for studies in immunology, cancer research, and inflammatory diseases.This antibody, raised in rabbits, is highly specific and sensitive for detecting NF-kB p65 protein in human samples, providing researchers with reliable and reproducible results in Western blot applications. Its validation in knockout models ensures accurate and specific targeting of NF-kB p65, making it a valuable tool for understanding the role of this transcription factor in various cellular processes.
With its ability to specifically detect NF-kB p65, this antibody is ideal for investigating the activation and regulation of NF-kB signaling in different cell types and tissues. By uncovering the mechanisms underlying NF-kB activation, researchers can gain insights into the development of novel therapies for inflammatory disorders, autoimmune diseases, and cancer.Overall, the KO Validated NF-kB p65 Rabbit Monoclonal Antibody (CAB19653) is a highly reliable and effective tool for researchers looking to explore the intricacies of NF-kB signaling and its implications in various disease contexts.
NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
5970
Clone Number:
ARC51086
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates, using NF-kB p65/RelA Rabbit mAb (CAB19653) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
