The KO Validated Lamin B1 Polyclonal Antibody (CAB1910) is a valuable tool for research involving Lamin B1, a key protein involved in nuclear envelope structure and function. This antibody, produced through immunization of rabbits, is highly specific and reactive with human samples, making it ideal for use in Western blot and immunohistochemistry applications.Lamin B1 is a crucial component of the nuclear lamina, providing structural support to the nucleus and playing a role in various cellular processes such as DNA replication, transcription, and nuclear envelope stability. Dysregulation of Lamin B1 has been implicated in various diseases, including progeria and certain cancers, making it an important target for biological research.
By using the KO Validated Lamin B1 Polyclonal Antibody, researchers can accurately detect and analyze Lamin B1 levels in different cell types and tissues, providing valuable insights into its physiological functions and potential implications in disease development. This antibody's validation for knockout studies ensures reliable and accurate results, making it a trusted reagent for studies in cell biology, developmental biology, and cancer research.
This gene encodes one of the two B-type lamin proteins and is a component of the nuclear lamina. A duplication of this gene is associated with autosomal dominant adult-onset leukodystrophy (ADLD). Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
4001
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Lamin B1 Rabbit pAb (CAB1910) at 1:1000 dilution.Jurkat cells were treated by Etoposide (25 uM) at 37℃ for 5 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
