The Jun/Jund Antibody (PAC06253) is a polyclonal antibody designed for research involving Jun and Jund proteins, key regulators of gene expression involved in cell growth, differentiation, and immune response modulation. Raised in rabbits, this antibody is highly reactive with human samples and is validated for use in various applications, including Western blotting and immunohistochemistry.Jun and Jund are members of the AP-1 transcription factor family, which play essential roles in regulating gene expression in response to various stimuli, including cytokines, growth factors, and stress signals. The Jun/Jund Antibody binds specifically to these proteins, allowing for detection and analysis in different cell types and tissues, making it ideal for studies in immunology, cancer research, and developmental biology.
Understanding the function and regulation of Jun and Jund proteins is crucial for deciphering their roles in normal cellular processes and disease states. Research using the Jun/Jund Antibody can provide valuable insights into the mechanisms underlying immune response modulation, cell proliferation, and differentiation, with potential implications for the development of novel therapeutic strategies targeting these pathways.
Antibody Name:
JUN/JUND Antibody (PACO06253)
Antibody SKU:
PACO06253
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, WB, IHC
Recommended Dilutions:
ELISA:1:10000, WB:1:500-1:2000, IHC:1:100-1:300
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Synthesized peptide derived from human AP-1 around the non-phosphorylation site of S73.
Form:
Liquid
Storage Buffer:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western Blot analysis of NIH-3T3 cells using AP-1/Jun D Polyclonal Antibody.
Western Blot analysis of HuvEc cells using AP-1/Jun D Polyclonal Antibody.
Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed:24623306). Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed:24623306).
NCBI Summary:
This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. [provided by RefSeq, Jul 2008]
