Human Triosephosphate Isomerase 1 (TPI1) ELISA Kit
The Human Triosephosphate Isomerase 1 (TPI1) ELISA Kit is a powerful tool for accurately measuring levels of TPI1 in human samples. This kit offers high sensitivity and specificity, providing dependable and consistent results for various research applications.Triosephosphate isomerase 1 is a key enzyme involved in glycolysis, a vital metabolic pathway that produces energy for cells. Dysregulation of TPI1 has been linked to various diseases, including cancer, metabolic disorders, and neurological conditions.
Therefore, this kit is essential for studying these diseases and developing potential treatments.With its reliable performance and ease of use, the Human TPI1 ELISA Kit is a valuable resource for researchers in the fields of biochemistry, cell biology, and medicine. Trust in this kit to deliver accurate and precise measurements of TPI1 levels, advancing your research efforts in understanding and combatting disease.
Product Name:
Human Triosephosphate Isomerase 1 (TPI1) ELISA Kit
Product Code:
HUDL02970
Size:
96 Assays
Target:
TPI1
Synonyms:
TIM, Triose-phosphate isomerase
Detection Method:
Sandwich
Range:
0.625-40ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
