The Human Sodium-Coupled Neutral Amino Acid Transporter 1 (SNAT1) ELISA Kit is specifically designed for the accurate detection of SNAT1 levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, providing reliable and reproducible results for a variety of research applications.SNAT1 is a critical protein involved in amino acid transport across cell membranes, playing a key role in nutrient uptake and cellular metabolism. Dysregulation of SNAT1 has been implicated in various diseases, including metabolic disorders, neurological conditions, and cancer.
As such, this ELISA kit serves as a valuable tool for researchers studying these diseases and exploring potential therapeutic interventions.With its advanced technology and user-friendly protocol, the Human SNAT1 ELISA Kit from AssayGenie is an essential resource for any researcher looking to investigate the role of SNAT1 in health and disease. Order yours today and unlock new insights into amino acid transport mechanisms and their implications for human health.
SLC38A1, NAT2, SAT1, ATA1, Solute Carrier Family 38 Member 1, N-system amino acid transporter 2, Amino acid transporter A1, System N amino acid transporter 1
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
