null

Human Sideroflexin 1 (SFXN1) ELISA Kit

SKU:
HUDL02662
Product Type:
ELISA Kit
Sensitivity:
27.9pg/mL
Range:
78.125-5000pg/mL
ELISA Type:
Sandwich
Synonyms:
TCC,Tricarboxylate carrier protein
Reactivity:
Human
Sample Type:
Tissue Homogenates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human Sideroflexin 1 (SFXN1) ELISA Kit

The Human SFXN1 (Sideroflexin 1) ELISA Kit is specifically designed for the precise and sensitive detection of SFXN1 levels in human samples such as serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it a valuable tool for various research applications.SFXN1 is a key protein involved in iron homeostasis and mitochondria function. Dysregulation of SFXN1 has been implicated in various diseases such as neurodegenerative disorders, iron-related disorders, and metabolic conditions.

Therefore, accurate measurement of SFXN1 levels can provide valuable insights into these conditions and aid in the development of potential treatments.Overall, the Human SFXN1 ELISA Kit offers researchers a reliable and efficient method for studying the role of SFXN1 in health and disease, ultimately contributing to advancements in the field of biomedical research.

Product Name:Human Sideroflexin 1 (SFXN1) ELISA Kit
Product Code:HUDL02662
Size:96 Assays
Target:SFXN1
Synonyms:TCC, Tricarboxylate carrier protein
Detection Method:Sandwich
Range:78.125-5000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

Related Products