null

Human Peptidase Inhibitor 3, Skin Derived (PI3) ELISA Kit

SKU:
HUDL02281
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P19957
Sensitivity:
0.132ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
SKALP,ESI,WAP3,WFDC14,Elafin,Skin-Derived Antileukoproteinase,Cementoin,Elastase-specific inhibitor,WAP four-disulfide core domain protein 14
Reactivity:
Human
Sample Type:
Serum, Plasma, Urine, Saliva, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human Peptidase Inhibitor 3, Skin Derived (PI3) ELISA Kit

The Human Peptidase Inhibitor 3 (PI3) ELISA Kit is specifically designed for the accurate detection of PI3 levels in human skin-derived samples, including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring dependable and reproducible results for a variety of research purposes.Peptidase Inhibitor 3 is a critical protein involved in regulating protease activity in the skin, playing a crucial role in maintaining skin health and function. Dysregulation of PI3 has been linked to various skin disorders, making it a valuable biomarker for studying these conditions and potentially developing new therapeutic interventions.

With its advanced technology and precise measurements, the Human Peptidase Inhibitor 3 (PI3) ELISA Kit is a valuable tool for researchers and clinicians working in dermatology, skin biology, and related fields. Gain valuable insights into PI3 levels and their implications for skin health with this reliable and easy-to-use assay kit.

Product Name:Human Peptidase Inhibitor 3, Skin Derived (PI3) ELISA Kit
Product Code:HUDL02281
Size:96 Assays
Target:PI3
Synonyms:SKALP, ESI, WAP3, WFDC14, Elafin, Skin-Derived Antileukoproteinase, Cementoin, Elastase-specific inhibitor, WAP four-disulfide core domain protein 14
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

Related Products