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Human Neuregulin 3 (NRG3) ELISA Kit

SKU:
HUDL02090
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P56975
Sensitivity:
0.063ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
Pro-NRG3,Pro-neuregulin-3, membrane-bound isoform
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Human Neuregulin 3 (NRG3) ELISA Kit

The Human Neuregulin-3 (NRG3) ELISA Kit from AssayGenie is a highly sensitive and specific assay for the accurate quantification of Neuregulin-3 levels in human samples such as serum, plasma, and cell culture supernatants. Neuregulin-3 is a key protein involved in various biological processes including nerve development, synapse formation, and heart development.This kit is designed to provide reliable and reproducible results, making it an essential tool for researchers studying conditions related to Neuregulin-3, such as schizophrenia, Alzheimer's disease, and cardiovascular disorders.

With its high sensitivity and specificity, this ELISA kit is suitable for a wide range of research applications and can help advance our understanding of Neuregulin-3 function and its potential role in disease pathology.

Product Name:Human Neuregulin 3 (NRG3) ELISA Kit
Product Code:HUDL02090
Size:96 Assays
Target:NRG3
Synonyms:Pro-NRG3, Pro-neuregulin-3, membrane-bound isoform
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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