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Human Myo Inositol Oxygenase (MIOX) ELISA Kit

SKU:
HUDL01855
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9UGB7
Sensitivity:
0.058ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
ALDRL6,KSP32,RSOR,Kidney-Specific Protein 32,Aldehyde Reductase(Aldose Reductase)Like 6,Renal-specific oxidoreductase,Myo-inositol oxygenase
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
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Description

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Human Myo Inositol Oxygenase (MIOX) ELISA Kit

The Human Myo-Inositol Oxygenase (MIOX) ELISA Kit is specifically designed for the precise measurement of MIOX levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications. MIOX is an enzyme that plays a critical role in myo-inositol metabolism, influencing various cellular processes and pathways.

Dysregulation of MIOX has been linked to conditions such as diabetes, kidney disease, and metabolic disorders, making it a valuable biomarker for studying these diseases and exploring potential treatment options. With its user-friendly protocol and reliable performance, the Human MIOX ELISA Kit is a valuable tool for researchers and clinicians looking to investigate the role of MIOX in health and disease.

Product Name:Human Myo Inositol Oxygenase (MIOX) ELISA Kit
Product Code:HUDL01855
Size:96 Assays
Target:MIOX
Synonyms:ALDRL6, KSP32, RSOR, Kidney-Specific Protein 32, Aldehyde Reductase(Aldose Reductase)Like 6, Renal-specific oxidoreductase, Myo-inositol oxygenase
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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