Human IgG4/Immunoglobulin heavy constant gamma 4 Polyclonal Antibody (CAB24655)
The Human IgG4 (Immunoglobulin Heavy Constant Gamma 4) Polyclonal Antibody is a valuable tool for researchers studying the IgG4 subtype of antibodies in human samples. This antibody, generated in rabbits, is highly specific and has been validated for use in various applications, including Western blot analysis.IgG4 antibodies play a critical role in the immune system, particularly in allergic reactions and autoimmune diseases. By targeting the IgG4 subtype, researchers can gain insight into the mechanisms of these conditions and potentially develop new therapeutic strategies.
This antibody allows for the detection and analysis of IgG4 in cell samples, making it a useful tool for studies in immunology and disease research.Overall, the Human IgG4 Polyclonal Antibody offers a reliable and effective way to study the IgG4 subtype of antibodies in human samples, providing valuable information for researchers working in fields such as immunology, allergy, and autoimmune diseases.
Product Name:
Human IgG4/Immunoglobulin heavy constant gamma 4 Polyclonal Antibody
SKU:
CAB24655
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 50-150 of human IgG4/Immunoglobulin heavy constant gamma 4 (P01861).
IGHG4; Ig gamma-4 chain C region; Human IgG4/Immunoglobulin heavy constant gamma 4
Positive Sample:
RPMI-8226,SH-SY5Y,Daudi,U-251 MG,K-562
Conjugate:
Unconjugated
Cellular Localization:
Secreted
Calculated MW:
44kDa
Observed MW:
36kDa
Immunoglobulin G (IgG), is one of the most abundant protein in human serum with normal levels between 8-17 mg/ml in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B-lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
Purification Method:
Affinity purification
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates, using Human IgG4/Immunoglobulin heavy constant gamma 4 Rabbit pAb (CAB24655) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
_WB_01__62317.1701346984.jpg?c=2&imbypass=on "Western blot analysis of various lysates, using Human IgG4/Immunoglobulin heavy constant gamma 4 Rabbit pAb (CAB24655) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.")
