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Human HS-TNF-α (High sensitive Tumor Necrosis Factor Alpha) ELISA Kit (AEFI01066)

SKU:
AEFI01066
Product Type:
ELISA Kit
Size:
96 Assays
Sensitivity:
1.875pg/ml
Range:
3.12-200pg/ml
€599
Frequently bought together:

Description

Product Name: Human HS-TNF-α(High sensitive Tumor Necrosis Factor Alpha) ELISA Kit
Alias: TNFα ELISA Kit, Tumor Necrosis Factor Alpha ELISA Kit, TNF-α ELISA Kit, DIF ELISA Kit, TNF-alpha ELISA Kit, TNFA ELISA Kit, TNFSF2 ELISA Kit
SKU: AEFI01066
Size: 96T
Species: Human
UniProt No: P01375
Sample Type: Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Method: Sandwich ELISA, Double Antibody
Detection Wavelength: OD450
Reaction Duration: 4 hours
Range: 3.12-200pg/ml
Sensitivity: 1.875pg/ml
Storage: 2-8°C(Sealed), Don't cryopreserve.
Specificity: Specifically binds with TNFα, no obvious cross reaction with other analogues.
Component Quantity Storage
ELISA Microplate (Dismountable) 8×12 strips Put the rest strips into a sealed foil bag with the desiccant. Stored for 1 month at 2-8°C; Stored for 6 months at -20°C
Lyophilized Standard 2 vials Put the rest standards into a desiccant bag. Stored for 1 month at 2-8°C; Stored for 6 months at -20°C
Biotin-labeled Antibody (Concentrated, 100X) 120 ul 2-8°C (Avoid Direct Light)
HRP-Streptavidin Conjugate (SABC, 100X) 120 ul
TMB Substrate 10 ml
Sample Dilution Buffer 20 ml 2-8°C
Antibody Dilution Buffer 10 ml
SABC Dilution Buffer 10 ml
Stop Solution 10 ml
Wash Buffer (Concentrated, 25X) 30 ml
Plate Sealer 5 pieces
Product Description 1 copy

Other materials and equipment required:

  • Microplate reader (wavelength: 450nm)
  • 37°C incubator (CO2 incubator for cell culture is not recommended.)
  • Automated plate washer or multi-channel pipette/5ml pipettor (for manual washing purpose)
  • Precision single (0.5-10μL, 5-50μL, 20-200μL, 200-1000μL) and multi-channel pipette with disposable tips(Calibration is required before use.)
  • Sterile tubes and Eppendorf tubes with disposable tips
  • Absorbent paper and loading slot
  • Deionized or distilled water
Recovery: Add a certain amount of TNFα into the sample. Calculate the recovery by comparing the measured value with the expected amount of TNFα in the sample.
Matrix Recovery range(%) Average(%)
serum(n=5) 86-100 94
EDTA plasma(n=5) 85-104 90
Heparin plasma(n=5) 89-101 96
Linearity: Dilute the sample with a certain amount of TNFα at 1:2, 1:4 and 1:8 to get the recovery range.
Sample 1:2 1:4 1:8
serum(n=5) 88-105% 85-95% 91-100%
EDTA plasma(n=5) 85-97% 86-99% 86-98%
Heparin plasma(n=5) 97-104% 90-97% 87-100%
Stability: Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.
ELISA kit(n=5) 37°C for 1 month 2-8°C for 6 months
Average(%) 80 95-100
Precision(%): Intra-Assay: CV<8%
Inter-Assay: CV<10%
Step 1: Add 100ul standard or sample into each well, seal the plate and statically incubate for 90 minutes at 37°C.
Washing: Wash the plate twice without immersing.
Step 2: Add 100ul biotin-antibody working solution, seal the plate and statically incubate for 60 minutes at 37°C.
Washing: Wash the plate three times and immerse for 1min each time.
Step 3: Add 100ul HRP-Streptavidin Conjugate (SABC) working solution, seal the plate and statically incubate for 30 minutes at 37°C.
Washing: Wash the plate five times and immerse for 1min each time.
Step 4: Add 90ul TMB substrate solution, seal the plate and statically incubate for 10-20 minutes at 37°C. (Accurate TMB visualization control is required.)
Step 5: Add 50ul stop solution. Read at 450nm immediately and calculate.

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