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Human Histidine Rich Glycoprotein (HRG) ELISA Kit

SKU:
HUDL01361
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P04196
Sensitivity:
6.8pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
HPRG,HRGP,Histidine-Proline Rich Glycoprotein,Thrombophilia Due To Elevated HRG
Reactivity:
Human
Sample Type:
Serum, Plasma And Other Biological Fluids
€649
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Description

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Human Histidine Rich Glycoprotein (HRG) ELISA Kit

The Human Histidine-Rich Glycoprotein (HRG) ELISA Kit is specifically designed for the accurate quantification of HRG levels in human serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, allowing for reliable and reproducible results in various research applications.HRG is a vital protein that plays a role in blood coagulation, angiogenesis, and immune response regulation. It is associated with various diseases such as cancer, cardiovascular diseases, and autoimmune disorders, making HRG a valuable biomarker for studying these conditions and developing potential therapeutic interventions.

This Human Histidine-Rich Glycoprotein (HRG) ELISA Kit provides researchers with a powerful tool to measure HRG levels accurately, ultimately advancing our understanding of the role of HRG in health and disease.

Product Name:Human Histidine Rich Glycoprotein (HRG) ELISA Kit
Product Code:HUDL01361
Size:96 Assays
Target:HRG
Synonyms:HPRG, HRGP, Histidine-Proline Rich Glycoprotein, Thrombophilia Due To Elevated HRG
Detection Method:Sandwich
Range:15.625-1,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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