Human GYG1 Recombinant Protein (RPPB1781)
- SKU:
- RPPB1781
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P46976
- Research Area:
- Enzymes
Description
| Product Name: | Human GYG1 Recombinant Protein |
| Product Code: | RPPB1781 |
| Size: | 20µg |
| Species: | Human |
| Target: | GYG1 |
| Synonyms: | Glycogenin-1, GYG1, GYG. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The GYG1 solution contains 20mM Tris-HCl buffer (pH 8.0), 1mM DTT and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MHHHHHHMAS MTGGQQMGRD LYDDDDKDRW GSMTDQAFVT LTTNDAYAKG ALVLGSSLKQ HRTTRRLVVL ATPQVSDSMR KVLETVFDEV IMVDVLDSGD SAHLTLMKRP ELGVTLTKLH CWSLTQYSKC VFMDADTLVL ANIDDLFDRE ELSAAPDPGW PDCFNSGVFV YQPSVETYNQ LLHLASEQGS FDGGDQGILN TFFSSWATTD IRKHLPFIYN LSSISIYSYL PAFKVFGASA KVVHFLGRVK PWNYTYDPKT KSVKSEAHDP NMTHPEFLIL WWNIFTTNVL PLLQQFGLVK DTCSYVNVED VSGAISHLSL GEIPAMAQPF VSSEERKERW EQGQADYMGA DSFDNIKRKL DTYLQ |
Glycogenin-1 (GYG1) is an enzyme involved in glycogen biosynthesis. GYG1 is the chief enzyme involved in glycogen polymerisation. Glycogenin-1 is vital for the function of self-glucosylates, using an inter-subunit mechanism, to form an oligosaccharide primer which acts as substrate for glycogen synthase. In addition, GYG1 has a role in regulating glycogen metabolism and the achievement of maximal glycogen levels in skeletal muscle. GYG1 mRNA and protein content and activity increase in the muscle during recovery from prolonged and exhaustive exercise. GYG1 is inactivated with glycogen catabolism which concurs with an increase in glycogenin gene expression as exercise and glycogenolysis advance. Glycogenin will remain covalently attached to the reducing end of the glycogen molecule.
GYG1 Human Recombinant fused with a 32 amino acid His-T7 tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 365 amino acids (1-333 a.a.) and having a molecular mass of 41.2kDa.The GYG1 is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | glycogenin: Self-glucosylates, via an inter-subunit mechanism, to form an oligosaccharide primer that serves as substrate for glycogen synthase. Defects in GYG1 are the cause of glycogen storage disease type 15 (GSD15). It is a metabolic disorder resulting in muscle weakness, associated with the glycogen depletion in skeletal muscle, and cardiac arrhythmia, associated with the accumulation of abnormal storage material in the heart. The skeletal muscle shows a marked predominance of slow-twitch, oxidative muscle fibers and mitochondrial proliferation. Belongs to the glycosyltransferase 8 family. Glycogenin subfamily. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 2.4.1.186; Transferase Chromosomal Location of Human Ortholog: 3q24 Cellular Component: cytosol; extracellular region; lysosomal lumen; membrane Molecular Function:glycogenin glucosyltransferase activity; protein binding Biological Process: glycogen biosynthetic process; glycogen catabolic process; neutrophil degranulation |
| NCBI Summary: | This gene encodes a member of the glycogenin family. Glycogenin is a glycosyltransferase that catalyzes the formation of a short glucose polymer from uridine diphosphate glucose in an autoglucosylation reaction. This reaction is followed by elongation and branching of the polymer, catalyzed by glycogen synthase and branching enzyme, to form glycogen. This gene is expressed in muscle and other tissues. Mutations in this gene result in glycogen storage disease XV. This gene has pseudogenes on chromosomes 1, 8 and 13 respectively. Alternatively spliced transcript variants encoding different isoforms have been identified.[provided by RefSeq, Sep 2010] |
| UniProt Code: | P46976 |
| NCBI GenInfo Identifier: | 13432151 |
| NCBI Gene ID: | 2992 |
| NCBI Accession: | P46976.4 |
| UniProt Secondary Accession: | P46976,Q6FHZ1, Q9UNV0, D3DNH0, D3DNH1, D3DNH2, |
| UniProt Related Accession: | P46976 |
| Molecular Weight: | 39kDa |
| NCBI Full Name: | Glycogenin-1 |
| NCBI Synonym Full Names: | glycogenin 1 |
| NCBI Official Symbol: | GYG1�� |
| NCBI Official Synonym Symbols: | GYG; GSD15�� |
| NCBI Protein Information: | glycogenin-1 |
| UniProt Protein Name: | Glycogenin-1 |
| UniProt Gene Name: | GYG1�� |
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