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Human Corin (CRN) ELISA Kit

SKU:
HUDL00685
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9Y5Q5
Sensitivity:
0.059ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
ATC2,CRN,Lrp4,TMPRSS10,Atrial Natriuretic Peptide-Converting Enzyme, Serine Protease,Heart-specific serine proteinase ATC2,Pro-ANP-converting enzyme
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€599
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Description

system_update_altDatasheet

Human Corin (CRN) ELISA Kit

The Human Corin (CRN) ELISA Kit is a must-have tool for researchers looking to accurately measure levels of corin in human samples including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reliable results for a variety of research applications.Corin is a vital enzyme involved in the regulation of blood pressure and cardiac function, making it a key player in cardiovascular health.

Dysregulation of corin has been linked to conditions such as hypertension and heart failure, highlighting the importance of studying its levels in different contexts.With the Human Corin (CRN) ELISA Kit, researchers can gain valuable insights into the role of corin in various diseases and conditions, paving the way for potential therapeutic interventions and advancements in the field of cardiovascular research.

Product Name:Human Corin (CRN) ELISA Kit
Product Code:HUDL00685
Size:96 Assays
Target:CRN
Synonyms:ATC2, CRN, Lrp4, TMPRSS10, Atrial Natriuretic Peptide-Converting Enzyme, Serine Protease, Heart-specific serine proteinase ATC2, Pro-ANP-converting enzyme
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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