The Human C4b (Complement Component 4b) ELISA Kit provides a precise and sensitive method for quantitatively measuring C4b levels in various biological samples.
Complement Component 4b plays a pivotal role in the immune system's complement pathway, particularly in the classical complement activation cascade. C4b is crucial for opsonization, immune complex clearance, and orchestrating host defense against pathogens. Understanding the levels of C4b is essential for evaluating complement system activity, immune responses, and immune-mediated diseases, including autoimmune disorders and infectious diseases. Accurate measurement of C4b is vital for elucidating its involvement in complement-mediated functions and immune surveillance mechanisms. Assay Genie's C4b ELISA Kit offers high sensitivity and specificity, ensuring reliable and reproducible results for research and clinical investigations. Manufactured with rigorous quality control measures, this kit consistently delivers robust performance and user-friendly protocols. Researchers can trust Assay Genie's C4b ELISA Kit for the accurate and dependable quantification of this critical complement component in their studies.
Product Name:
Human C4b (Complement Component 4b) ELISA Kit
SKU:
AEES00095
Target:
Human C4b (Complement Component 4b)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human C4b. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human C4b and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human C4b, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human C4b. You can calculate the concentration of Human C4b in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-107
96-106
96-104
Average (%)
99
99
101
1:4
Range (%)
92-104
85-96
102-108
Average (%)
96
90
105
1:8
Range (%)
99-110
96-109
82-93
Average (%)
103
103
90
1:16
Range (%)
87-101
86-95
98-111
Average (%)
95
91
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-98
93
EDTA plasma (n=5)
93-108
101
Cell culture media (n=5)
83-96
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.25
10.6
44.19
4.12
10.49
41.9
Standard deviation
0.24
0.5
1.83
0.22
0.49
1.93
C V (%)
5.65
4.72
4.14
5.34
4.67
4.61
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human C4b concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human C4b in samples. No significant cross-reactivity or interference between Human C4b and analogues was observed.
