The HLA-DRA Polyclonal Antibody (CAB11787) is a powerful tool for researchers studying the human leukocyte antigen HLA-DRA, a key player in the immune response. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By targeting the HLA-DRA protein, this antibody allows for precise detection and analysis in a variety of cell types, making it well-suited for investigations in immunology, autoimmunity, and infectious diseases.HLA-DRA is a critical component of the major histocompatibility complex (MHC) class II molecules, responsible for presenting antigen peptides to T cells and initiating adaptive immune responses.
Its role in antigen presentation and immune recognition underscores its importance in understanding immune function and the development of immune-based therapies. Research involving HLA-DRA has implications for a wide range of diseases, including autoimmune disorders, infectious diseases, and cancer, making this polyclonal antibody an essential tool for advancing our knowledge in these areas.
Product Name:
HLA-DRA Rabbit Polyclonal Antibody
SKU:
CAB11787
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 26-216 of human HLA-DRA (NP_061984.2).
Cell membrane, Endoplasmic reticulum membrane, Endosome membrane, Golgi apparatus, Late endosome membrane, Lysosome membrane, Single-pass type I membrane protein, trans-Golgi network membrane.
Calculated MW:
29kDa
Observed MW:
36kDa
HLA-DRA is one of the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha and a beta chain, both anchored in the membrane. This molecule is expressed on the surface of various antigen presenting cells such as B lymphocytes, dendritic cells, and monocytes/macrophages, and plays a central role in the immune system and response by presenting peptides derived from extracellular proteins, in particular, pathogen-derived peptides to T cells. The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. DRA does not have polymorphisms in the peptide binding part and acts as the sole alpha chain for DRB1, DRB3, DRB4 and DRB5.
Purification Method:
Affinity purification
Gene ID:
3122
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using HLA-DRA Rabbit pAb (CAB11787) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
