The HLA-C Antibody (PAC05446) is a polyclonal antibody specifically designed for research involving the HLA-C molecule, a key player in the immune system's recognition of foreign invaders and self-cells. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in various applications, including Western blotting.HLA-C, a member of the human leukocyte antigen (HLA) system, plays a crucial role in the immune response by presenting antigens to immune cells and regulating immune function. Understanding the function and expression of HLA-C is important for research in immunology, transplantation, infection, and autoimmune diseases.
The HLA-C Antibody enables researchers to detect and analyze HLA-C expression in different cell types, providing valuable insights into its role in immune responses and diseases.With its specificity and reliability, the HLA-C Antibody is an essential tool for scientists conducting studies on the immune system and its involvement in various health conditions. Whether investigating immune responses in cancer, infectious diseases, or autoimmune disorders, this antibody facilitates the exploration of HLA-C's impact on disease progression and immune system modulation.
Immunohistochemistry of paraffin-embedded human lymph node tissue using PACO54462 at dilution of 1:100.
Immunofluorescence staining of HepG2 cells with PACO54462 at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
Involved in the presentation of foreign antigens to the immune system.
Synonyms:
HLA class I histocompatibility antigen, Cw-3 α chain (MHC class I antigen Cw*3), HLA-C, HLAC
UniProt Protein Function:
Involved in the presentation of foreign antigens to the immune system.
UniProt Protein Details:
NCBI Summary:
HLA-C belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Over one hundred HLA-C alleles have been described [provided by RefSeq, Jul 2008]
