HLA-B Rabbit Polyclonal Antibody (CAB1285)
- SKU:
- CAB1285
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Description
Product Name: | HLA-B Rabbit Polyclonal Antibody |
SKU: | CAB1285 |
Size: | 20uL, 100uL |
Isotype: | IgG |
Host Species: | Rabbit |
Reactivity: | Human,Mouse,Rat |
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HLA-B (NP_005505.2). |
Sequence: | MLVM APRT VLLL LSAA LALT ETWA GSHS MRYF YTSV SRPG RGEP RFIS VGYV DDTQ FVRF DSDA ASPR EEPR APWI EQEG PEYW DRNT QIYK AQAQ TDRE |
Tested Applications: | WB IHC-P IF/ICC ELISA |
Recommended Dilution: | WB,1:1000 - 1:2000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200 |
Synonyms: | AS; HLAB; B-4901; HLA-B |
Positive Sample: | U-251 MG,Hep G2,A549,MCF7 |
Conjugate: | Unconjugated |
Cellular Localization: | Membrane, Single-pass type I membrane protein. |
Calculated MW: | 40kDa |
Observed MW: | 41kDa |
HLA-B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exon 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-B alleles have been described.
Purification Method: | Affinity purification |
Gene ID: | 3106 |
Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3. |