The Histone H2A.Z Polyclonal Antibody (CAB6614) is a valuable tool for research in epigenetics and chromatin biology. Histone H2A.Z is a variant of the histone protein H2A that plays a crucial role in regulating gene expression and chromatin structure. This antibody, developed in rabbits, shows high reactivity with human samples and has been validated for use in Western blot applications.Histone H2A.Z is involved in key cellular processes such as transcriptional regulation, DNA repair, and genome stability. Dysregulation of H2A.Z has been linked to various diseases, including cancer and neurological disorders. By targeting H2A.Z, researchers can gain insights into the molecular mechanisms underlying these conditions and potentially identify new therapeutic targets.
This antibody binds specifically to the Histone H2A.Z protein, enabling precise detection and analysis in a variety of cell types and experimental conditions. Its versatility and reliability make it an essential tool for studying epigenetic modifications and their impact on gene expression. Researchers in the fields of molecular biology, genetics, and cancer research will benefit from using this antibody to further their understanding of histone biology and its implications for human health and disease.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent member of the histone H2A family that is distinct from other members of the family. Studies in mice have shown that this particular histone is required for embryonic development and indicate that lack of functional histone H2A leads to embryonic lethality.
Purification Method:
Affinity purification
Gene ID:
3015
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using [KO Validated] Histone H2A.Z Rabbit pAb (CAB6614) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
