The Hist1H2ac Antibody (PAC061718) offered by AssayGenie is a high-quality polyclonal antibody designed for research involving the Hist1H2ac protein. This antibody, raised in rabbits, is highly reactive with human samples and has been validated for use in Western blot applications. Hist1H2ac is a histone protein that plays a crucial role in the packaging of DNA into chromatin. By targeting the Hist1H2ac protein, researchers can study the epigenetic regulation of gene expression, chromatin structure, and overall genome stability. This antibody enables the detection and analysis of Hist1H2ac in various cell types, making it ideal for studies in epigenetics, gene regulation, and cancer research.
Understanding the function and regulation of Hist1H2ac is essential for unraveling the complexities of chromatin biology and its impact on various cellular processes. With the Hist1H2ac Antibody from AssayGenie, researchers can delve deeper into the mechanisms governing gene expression and chromatin remodeling, ultimately advancing our knowledge of epigenetics and its implications in health and disease.
IHC image of PACO61718 diluted at 1:400 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of A549 cells with PACO61718 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Synonyms:
Histone H2A type 1-C (Histone H2A/l), HIST1H2AC, H2AFL
UniProt Protein Function:
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
UniProt Protein Details:
NCBI Summary:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2A family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]
