High Sensitivity Chemiluminescent Substrate (ECL) Detection Kit (AGEL3539)

Chemiluminescence is the emission of light during a chemical reaction. This process is often used in biological assays like Western blots and ELISA to detect the presence of proteins. The light emitted can be measured to quantify the amount of protein present. In the case of horseradish peroxidase (HRP), chemiluminescent substrates react with HRP to produce light. The Excellent Chemiluminescent Substrate Kit from Assay Genie provides a high-intensity, long-lasting signal that allows for the detection of proteins with picogram-level sensitivity.

1. Block nonspecific sites with Blocking Reagent for 20-60 minutes at room temperature.

2. Incubate blot with the primary antibody dilution for 1 hour at RT or overnight at 2-8°C.

3. Wash the membrane thoroughly and incubate with HRP-conjugate for 1 hour.

4. Prepare the Working Solution by mixing equal parts of Substrate A and B. Incubate blot for 5 minutes.

5. Place the blot in a film cassette for exposure.

What is chemiluminescence?

Chemiluminescence is the emission of light as a result of a chemical reaction. It is often used in molecular biology assays to detect specific proteins through light emission.

How sensitive is the Excellent Chemiluminescent Substrate Kit?

This kit provides low picogram sensitivity, allowing for the detection of small amounts of protein in both Western blot and ELISA applications.

How should I store the kit?

The kit should be stored at 2~8°C. Avoid freeze-thaw cycles to maintain performance.

What is the stability of the working solution?

The working solution is stable for up to 8 hours at room temperature once prepared.