The HHAT Polyclonal Antibody (CAB15171) is a vital tool for research involving the Hedgehog acyltransferase (HHAT) protein, which plays a key role in the Hedgehog signaling pathway that regulates cell growth and differentiation. This antibody, produced in rabbits, exhibits high specificity and sensitivity towards human HHAT protein in various experimental applications, particularly in Western blot analysis.The HHAT protein is essential for the lipid modification of Hedgehog ligands, which are signaling molecules involved in various developmental processes, including embryogenesis and tissue regeneration. Dysregulation of the Hedgehog pathway, including alterations in HHAT activity, has been implicated in numerous diseases, such as cancer and developmental disorders.
Therefore, studying HHAT function using this antibody can provide valuable insights into the mechanisms underlying these pathologies.Researchers focused on understanding the Hedgehog signaling pathway and its implications in disease pathogenesis will find this HHAT Polyclonal Antibody (CAB15171) indispensable for their investigations. Its ability to specifically detect HHAT protein in human samples makes it a versatile tool for studies aimed at elucidating the role of Hedgehog signaling in various biological processes and disease contexts.
Product Name:
HHAT Rabbit Polyclonal Antibody
SKU:
CAB15171
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HHAT (NP_060664.2).
Skinny hedgehog' (SKI1) encodes an enzyme that acts within the secretory pathway to catalyze amino-terminal palmitoylation of 'hedgehog' (see MIM 600725).
Purification Method:
Affinity purification
Gene ID:
55733
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using HHAT Rabbit pAb (CAB15171) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
