The Hemoglobin Subunit Alpha Rabbit Monoclonal Antibody (CAB9293) is a cutting-edge tool designed for research involving the hemoglobin subunit alpha protein. This antibody, developed using rabbit monoclonal technology, is highly specific and reactive with human samples, making it an ideal choice for a wide range of applications in biochemistry and molecular biology.The hemoglobin subunit alpha protein is a key component of hemoglobin, the molecule responsible for transporting oxygen in red blood cells. Studying this protein can provide valuable insights into conditions such as anemia, sickle cell disease, and other blood disorders. The CAB9293 antibody enables precise detection and analysis of the hemoglobin subunit alpha protein in various cell types, allowing researchers to delve deeper into its role in health and disease.
With its high specificity and sensitivity, the CAB9293 antibody is well-suited for techniques such as Western blotting, immunofluorescence, and immunohistochemistry. Its reliable performance and consistent results make it a trusted reagent for studies in hematology, biochemistry, and clinical research. By using the CAB9293 antibody, scientists can advance their understanding of hemoglobin biology and potentially uncover new therapeutic targets for blood-related disorders.
The human alpha globin gene cluster located on chromosome 16 spans about 30 kb and includes seven loci: 5'- zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 - alpha-1 - theta - 3'. The alpha-2 (HBA2) and alpha-1 (HBA1) coding sequences are identical. These genes differ slightly over the 5' untranslated regions and the introns, but they differ significantly over the 3' untranslated regions. Two alpha chains plus two beta chains constitute HbA, which in normal adult life comprises about 97% of the total hemoglobin; alpha chains combine with delta chains to constitute HbA-2, which with HbF (fetal hemoglobin) makes up the remaining 3% of adult hemoglobin. Alpha thalassemias result from deletions of each of the alpha genes as well as deletions of both HBA2 and HBA1; some nondeletion alpha thalassemias have also been reported.
Purification Method:
Affinity purification
Gene ID:
3039
Clone Number:
ARC1510
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from Rat liver, using Hemoglobin subunit alpha (HBA1) (HBA1) Rabbit mAb (CAB9293) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
