Description
GNAS Antibody (CAB5546)
The GNAS Antibody (CAB5546) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific for human samples and is validated for use in Western blot applications. It recognizes the GNAS protein, allowing for the detection and analysis of GNAS expression in a variety of cell types.GNAS, also known as the guanine nucleotide-binding protein G(s) subunit alpha, plays a crucial role in regulating intracellular signaling cascades that control processes such as cell proliferation, differentiation, and metabolism. Dysregulation of GNAS has been linked to a variety of diseases, including endocrine disorders and certain types of cancer.
This antibody is validated for use in WB, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
| Product Name: | GNAS Antibody |
| SKU: | CAB5546 |
| Size: | 20μL, 100μL |
| Reactivity: | Human, Mouse, Rat |
| Conjugate: | Unconjugated |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. | ||||||||
| Sequence: | MGCL GNSK TEDQ RNEE KAQR EANK KIEK QLQK DKQV YRAT HRLL LLGA GESG KSTI VKQM RILH VNGF NGEG GEED PQAA RSNS DGEK ATKV QDIK NNLK | ||||||||
| Tested Applications: | WB IF/ICC IP ELISA | ||||||||
| Recommended Dilution: |
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| Synonyms: | AHO, GSA, GSP, POH, GPSA, NESP, SCG6, SgVI, GNAS1, PITA3, C20orf45, GNAS |
| Positive Sample: | HepG2, HeLa, Mouse lung, Mouse brain, Rat brain |
| Cellular Localization: | Cell Membrane By Similarity; Lipid-Anchor By Similarity. |
| Calculated MW: | 28kDa/44kDa/45kDa/77kDa/109kDa/111kDa |
| Observed MW: | 50kDa |
This locus has a highly complex imprinted expression pattern. It gives rise to maternally, paternally, and biallelically expressed transcripts that are derived from four alternative promoters and 5' exons. Some transcripts contain a differentially methylated region (DMR) at their 5' exons, and this DMR is commonly found in imprinted genes and correlates with transcript expression. An antisense transcript is produced from an overlapping locus on the opposite strand. One of the transcripts produced from this locus, and the antisense transcript, are paternally expressed noncoding RNAs, and may regulate imprinting in this region. In addition, one of the transcripts contains a second overlapping ORF, which encodes a structurally unrelated protein - Alex. Alternative splicing of downstream exons is also observed, which results in different forms of the stimulatory G-protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Multiple transcript variants encoding different isoforms have been found for this gene. Mutations in this gene result in pseudohypoparathyroidism type 1a, pseudohypoparathyroidism type 1b, Albright hereditary osteodystrophy, pseudopseudohypoparathyroidism, McCune-Albright syndrome, progressive osseus heteroplasia, polyostotic fibrous dysplasia of bone, and some pituitary tumors.
| Purification Method | Affinity purification |
| Gene ID | 2778 |
| RRID | AB_2766327 |
| Buffer Information | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3. |
 | Western blot analysis of various lysates using GNAS Rabbit pAb (CAB5546) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s. |
 | Immunofluorescence analysis of L929 cells using GNAS Rabbit pAb (CAB5546) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
 | Immunoprecipitation analysis of 200 μg extracts of Mouse brain cells using 3 μg GNAS antibody (CAB5546). Western blot was performed from the immunoprecipitate using GNAS antibody (CAB5546) at a dilution of 1:1000. |
