The GNAS Polyclonal Antibody (CAB5546) is a valuable tool for researchers studying GNAS, a key gene involved in various cellular signaling pathways. This antibody, produced in rabbits, is highly specific for human samples and is validated for use in Western blot applications. It recognizes the GNAS protein, allowing for the detection and analysis of GNAS expression in a variety of cell types.GNAS, also known as the guanine nucleotide-binding protein G(s) subunit alpha, plays a crucial role in regulating intracellular signaling cascades that control processes such as cell proliferation, differentiation, and metabolism. Dysregulation of GNAS has been linked to a variety of diseases, including endocrine disorders and certain types of cancer.
By studying GNAS expression and activity, researchers can gain important insights into the molecular mechanisms underlying these conditions.The GNAS Polyclonal Antibody is an essential tool for investigating the function of GNAS in both normal physiology and disease states. Its high specificity and sensitivity make it ideal for use in immunology, cancer research, and other fields where understanding GNAS signaling is key. By elucidating the role of GNAS, researchers can pave the way for the development of targeted therapies and diagnostic tools for GNAS-related disorders.
Product Name:
GNAS Rabbit Polyclonal Antibody
SKU:
CAB5546
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human GNAS (NP_000507.1).
Cell membrane By similarity; Lipid-anchor By similarity.
Calculated MW:
28kDa/44kDa/45kDa/77kDa/109kDa/111kDa
Observed MW:
50kDa
This locus has a highly complex imprinted expression pattern. It gives rise to maternally, paternally, and biallelically expressed transcripts that are derived from four alternative promoters and 5' exons. Some transcripts contain a differentially methylated region (DMR) at their 5' exons, and this DMR is commonly found in imprinted genes and correlates with transcript expression. An antisense transcript is produced from an overlapping locus on the opposite strand. One of the transcripts produced from this locus, and the antisense transcript, are paternally expressed noncoding RNAs, and may regulate imprinting in this region. In addition, one of the transcripts contains a second overlapping ORF, which encodes a structurally unrelated protein - Alex. Alternative splicing of downstream exons is also observed, which results in different forms of the stimulatory G-protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Multiple transcript variants encoding different isoforms have been found for this gene. Mutations in this gene result in pseudohypoparathyroidism type 1a, pseudohypoparathyroidism type 1b, Albright hereditary osteodystrophy, pseudopseudohypoparathyroidism, McCune-Albright syndrome, progressive osseus heteroplasia, polyostotic fibrous dysplasia of bone, and some pituitary tumors.
Purification Method:
Affinity purification
Gene ID:
2778
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using GNAS Rabbit pAb (CAB5546) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
