The GLDC Polyclonal Antibody (CAB10006) is specifically designed for research involving the GLDC protein, which plays a crucial role in glycine degradation and amino acid metabolism. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding to the GLDC protein, it allows for the detection and analysis of GLDC in various cell types, making it an essential tool for studies in metabolism, biochemistry, and genetic disorders.GLDC, also known as glycine decarboxylase, is a key enzyme involved in the breakdown of glycine, an essential amino acid required for various cellular processes.
Dysregulation of GLDC activity has been implicated in a range of metabolic disorders, making it a significant target for research in fields such as metabolic diseases, neurology, and developmental biology. Understanding the function and regulation of GLDC is essential for advancing our knowledge of these conditions and developing potential therapies in the future.
Product Name:
GLDC Polyclonal Antibody
SKU:
CAB24541
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 580-749 of human GLDC (NP_000161.2).
Degradation of glycine is brought about by the glycine cleavage system, which is composed of four mitochondrial protein components: P protein (a pyridoxal phosphate-dependent glycine decarboxylase), H protein (a lipoic acid-containing protein), T protein (a tetrahydrofolate-requiring enzyme), and L protein (a lipoamide dehydrogenase). The protein encoded by this gene is the P protein, which binds to glycine and enables the methylamine group from glycine to be transferred to the T protein. Defects in this gene are a cause of nonketotic hyperglycinemia (NKH).
Purification Method:
Affinity purification
Gene ID:
2731
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates, using GLDC Rabbit pAb (CAB24541) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
