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GLDC Polyclonal Antibody (CAB24541)

SKU:
CAB24541
$155
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Description

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Product Name:GLDC Polyclonal Antibody
SKU:CAB24541
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 580-749 of human GLDC (NP_000161.2).
Sequence:HPFV PLDQ AQGY QQLF RELE KDLC ELTG YDQV CFQP NSGA QGEY AGLA TIRA YLNQ KGEG HRTV CLIP KSAH GTNP ASAH MAGM KIQP VEVD KYGN IDAV HLKA MVDK HKEN LAAI MITY PSTN GVFE ENIS DVCD LIHQ HGGQ VYLD GANM NAQV GICR PGDF GSDV SH
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:WB,1:500 - 1:1000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200
Synonyms:GLDC; GCE; GCSP; HYGN1; glycine decarboxylase
Positive Sample:BeWo,Hep G2,U-251 MG (Low expression control),Mouse liver,Rat kidney,Rat liver
Conjugate:Unconjugated
Cellular Localization:Mitochondrion.
Calculated MW:112kDa
Observed MW:113kDa

Degradation of glycine is brought about by the glycine cleavage system, which is composed of four mitochondrial protein components: P protein (a pyridoxal phosphate-dependent glycine decarboxylase), H protein (a lipoic acid-containing protein), T protein (a tetrahydrofolate-requiring enzyme), and L protein (a lipoamide dehydrogenase). The protein encoded by this gene is the P protein, which binds to glycine and enables the methylamine group from glycine to be transferred to the T protein. Defects in this gene are a cause of nonketotic hyperglycinemia (NKH).

Purification Method:Affinity purification
Gene ID:2731
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates, using GLDC Rabbit pAb (CAB24541) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.

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