The GINS1 Polyclonal Antibody (PAC058128) is a valuable tool for researchers studying GINS1, a key component of the GINS complex involved in DNA replication. This antibody, raised in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to the GINS1 protein, this antibody allows for accurate detection and analysis in various cell types, making it ideal for research in molecular biology and cancer biology.GINS1 plays a crucial role in DNA replication, making it a target of interest for studies on cell proliferation, cancer development, and genetic disorders.
Understanding the function of GINS1 is essential for unraveling the mechanisms of DNA replication and identifying potential therapeutic targets for conditions related to abnormal cell division. With its high specificity and reliability, the GINS1 Polyclonal Antibody is a valuable tool for scientists investigating the intricate processes of DNA replication and cell growth.
Antibody Name:
GINS1 Antibody (PACO58128)
Antibody SKU:
PACO58128
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:2000-1:10000, IHC:1:200-1:500
Species Reactivity:
Human
Immunogen:
Recombinant Human DNA replication complex GINS protein PSF1 protein (1-196AA)
IHC image of PACO58128 diluted at 1:400 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of PACO58128 diluted at 1:400 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:
The GINS complex plays an essential role in the initiation of DNA replication, and progression of DNA replication forks. GINS complex seems to bind preferentially to single-stranded DNA. GINS1 is essential for function.
Synonyms:
DNA replication complex GINS protein PSF1 (GINS complex subunit 1), GINS1, KIAA0186 PSF1
UniProt Protein Function:
GINS1: The GINS complex plays an essential role in the initiation of DNA replication, and progression of DNA replication forks. GINS complex seems to bind preferentially to single- stranded DNA. GINS1 is essential for function. Belongs to the GINS1/PSF1 family.Protein type: Motility/polarity/chemotaxis; DNA replicationChromosomal Location of Human Ortholog: 20p11.21Cellular Component: cytoplasm; nucleoplasm; nucleusMolecular Function: 3'-5' DNA helicase activityBiological Process: DNA strand elongation during DNA replication
UniProt Protein Details:
NCBI Summary:
The yeast heterotetrameric GINS complex is made up of Sld5 (GINS4; MIM 610611), Psf1, Psf2 (GINS2; MIM 610609), and Psf3 (GINS3; MIM 610610). The formation of the GINS complex is essential for the initiation of DNA replication in yeast and Xenopus egg extracts (Ueno et al., 2005 [PubMed 16287864]).[supplied by OMIM, Mar 2008]
