The GCSH Polyclonal Antibody (CAB13695) is a cutting-edge tool for researchers studying GCSH, an enzyme involved in leucine catabolism and energy metabolism. This high-quality antibody, produced in rabbits, exhibits strong reactivity with human samples and is validated for use in Western blotting applications. By targeting the GCSH protein, this antibody allows for precise detection and analysis in a variety of cell types, making it an invaluable resource for studies in metabolism, energy regulation, and mitochondrial function.
GCSH plays a crucial role in maintaining normal metabolic processes by breaking down leucine, an essential amino acid, and producing energy in the form of ATP. Dysregulation of GCSH activity has been linked to metabolic disorders, including maple syrup urine disease, making it a compelling target for further research and therapeutic development. Investigating the function of GCSH with the help of this antibody may lead to a better understanding of metabolic diseases and the development of targeted treatments to address them.
Product Name:
GCSH Rabbit Polyclonal Antibody
SKU:
CAB13695
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-173 of human GCSH (NP_004474.2).
Degradation of glycine is brought about by the glycine cleavage system, which is composed of four mitochondrial protein components: P protein (a pyridoxal phosphate-dependent glycine decarboxylase), H protein (a lipoic acid-containing protein), T protein (a tetrahydrofolate-requiring enzyme), and L protein (a lipoamide dehydrogenase). The protein encoded by this gene is the H protein, which transfers the methylamine group of glycine from the P protein to the T protein. Defects in this gene are a cause of nonketotic hyperglycinemia (NKH). Two transcript variants, one protein-coding and the other probably not protein-coding,have been found for this gene. Also, several transcribed and non-transcribed pseudogenes of this gene exist throughout the genome.
Purification Method:
Affinity purification
Gene ID:
2653
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates, using GCSH Rabbit pAb (CAB13695) at 1:700 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
