The GCLM Polyclonal Antibody (CAB5314) is a valuable tool for researchers studying the glutamate-cysteine ligase modifier subunit (GCLM) protein. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By targeting the GCLM protein, this antibody allows for the detection and analysis of GCLM in a variety of cell types, making it an essential tool for research in molecular biology and biochemistry.GCLM is a key enzyme in the glutathione synthesis pathway, playing a critical role in antioxidant defense and cellular detoxification.
Dysregulation of GCLM has been implicated in various diseases, including cancer, neurodegenerative disorders, and metabolic syndromes. By understanding the expression and function of GCLM, researchers can gain insights into the pathogenesis of these conditions and potentially identify new therapeutic targets.Overall, the GCLM Polyclonal Antibody (CAB5314) provides researchers with a reliable tool for investigating the role of GCLM in health and disease, ultimately contributing to advancements in medical research and drug development.
Product Name:
GCLM Rabbit Polyclonal Antibody
SKU:
CAB5314
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-274 of human GCLM (NP_002052.1).
Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. Alternative splicing results in multiple transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
2730
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using GCLM antibody (CAB5314) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
