The GCHFR Polyclonal Antibody (PAC022357) is a valuable tool for researchers studying GCHFR, an enzyme involved in regulating the production of tetrahydrobiopterin, a critical cofactor in the synthesis of neurotransmitters and nitric oxide. This antibody, produced in rabbits, exhibits high specificity for human samples and is validated for use in Western blot applications. By binding to the GCHFR protein, this antibody enables accurate detection and analysis in various cell types, making it ideal for studies in neuroscience, metabolism, and cardiovascular research.
GCHFR plays a key role in maintaining optimal levels of tetrahydrobiopterin, which is essential for the production of neurotransmitters like dopamine, serotonin, and nitric oxide. Dysregulation of GCHFR activity has been linked to neurodegenerative diseases, cardiovascular disorders, and metabolic disorders. Therefore, understanding the function of GCHFR is crucial for developing therapeutic strategies targeting these conditions and advancing our knowledge of biopterin metabolism.
Antibody Name:
GCHFR Antibody (PACO22357)
Antibody SKU:
PACO22357
Size:
100ul
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:2000-1:10000, IHC:1:50-1:100
Species Reactivity:
Human, Mouse
Immunogen:
Synthesized peptide derived from internal of human GCHFR.
Form:
Liquid
Storage Buffer:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using GCHFR antibody.
Background:
Mediates tetrahydrobiopterin inhibition of GTP cyclohydrolase 1. This inhibition is reversed by L-phenylalanine.
GCHFR: Mediates tetrahydrobiopterin inhibition of GTP cyclohydrolase 1. This inhibition is reversed by L-phenylalanine. Belongs to the GFRP family.Protein type: InhibitorChromosomal Location of Human Ortholog: 15q15Cellular Component: cytoplasm; cytosol; dendrite; melanosome; nuclear membrane; nucleusMolecular Function: protein bindingBiological Process: neurotransmitter metabolic process; nitric oxide biosynthetic process; regulation of nitric-oxide synthase activity
UniProt Protein Details:
NCBI Summary:
GTP cyclohydrolase I feedback regulatory protein binds to and mediates tetrahydrobiopterin inhibition of GTP cyclohydrolase I. The regulatory protein, GCHFR, consists of a homodimer. It is postulated that GCHFR may play a role in regulating phenylalanine metabolism in the liver and in the production of biogenic amine neurotransmitters and nitric oxide. [provided by RefSeq, Jul 2008]
