The GAPDH Rabbit Monoclonal Antibody (CAB19056) is a powerful tool for researchers studying the well-known housekeeping protein GAPDH. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in a variety of applications, including Western blotting. By specifically binding to the GAPDH protein, this antibody enables precise detection and analysis in various cell types, making it an indispensable tool for investigations in areas such as biochemistry, cell biology, and molecular biology.GAPDH, or glyceraldehyde-3-phosphate dehydrogenase, is a key enzyme involved in glycolysis and plays a crucial role in cellular metabolism.
Its versatile functions go beyond energy production, with implications in various cellular processes such as apoptosis, DNA repair, and gene expression regulation. Research on GAPDH has shed light on its involvement in diseases like cancer, neurodegenerative disorders, and diabetes, making it a target of interest for drug development and biomarker discovery.By utilizing the GAPDH Rabbit Monoclonal Antibody (CAB19056), scientists can explore the intricate roles of GAPDH in cellular physiology and pathology, paving the way for novel insights and potential therapeutic strategies in a wide range of scientific disciplines.
Product Name:
GAPDH Rabbit Monoclonal Antibody
SKU:
CAB19056
Size:
100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 4-335 of human GAPDH (NP_002037.2).
This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
2597
Clone Number:
ARC50888
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates, using GAPDH Rabbit mAb (CAB19056) at 1:100000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
