The GALR2 Polyclonal Antibody (CAB17577) is a valuable tool for researchers studying GALR2, a G protein-coupled receptor known for its role in modulating neuroendocrine functions. This antibody, developed in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding to the GALR2 protein, this antibody enables precise detection and analysis in a variety of cell types, making it a versatile option for studies in neuroscience and endocrinology research.GALR2, or galanin receptor 2, is involved in regulating the release of neurotransmitters and hormones, impacting processes such as pain perception, appetite regulation, and stress responses.
Research on GALR2 is particularly relevant in understanding conditions like chronic pain, eating disorders, and mood disorders. By elucidating the mechanisms of GALR2 activity, researchers can gain insights into potential therapeutic targets for these complex conditions, ultimately paving the way for innovative treatment strategies.
Product Name:
GALR2 Rabbit Polyclonal Antibody
SKU:
CAB17577
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 50-150 of human GALR2 (NP_003848.1).
Galanin is an important neuromodulator present in the brain, gastrointestinal system, and hypothalamopituitary axis. It is a 30-amino acid non-C-terminally amidated peptide that potently stimulates growth hormone secretion, inhibits cardiac vagal slowing of heart rate, abolishes sinus arrhythmia, and inhibits postprandial gastrointestinal motility. The actions of galanin are mediated through interaction with specific membrane receptors that are members of the 7-transmembrane family of G protein-coupled receptors. GALR2 interacts with the N-terminal residues of the galanin peptide. The primary signaling mechanism for GALR2 is through the phospholipase C/protein kinase C pathway (via Gq), in contrast to GALR1, which communicates its intracellular signal by inhibition of adenylyl cyclase through Gi. However, it has been demonstrated that GALR2 couples efficiently to both the Gq and Gi proteins to simultaneously activate 2 independent signal transduction pathways.
Purification Method:
Affinity purification
Gene ID:
8811
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using GALR2 Rabbit pAb (CAB17577) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 2min.
